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KMID : 0359319890290040525
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Korean Journal of Veterinary Research
1989 Volume.29 No. 4 p.525 ~ p.530
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Enumeration of Korean native goat erythrocytes ( KGRBC ) - rosette forming cells in peripheral blood of Korean cattle
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김동훈/Kim, Dong Hoon
송희종/정기수/김년수/강명대/Song, Hee Jong/Cheong, Ki Soo/Kim, Neun Su/Kang, Myoung Dai
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Abstract
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In order to enumerate the T-lymphocytes in bovine peripheral blood lymphocytes (PBL) by E rosette assay, KGRBC were treated with various concentrations of 2-aminoethyli-sothiouronium bromide(AET) and dextran(Dex), singly or in combination. To further standardize the assay, optimum concentration of AET- and/or Dex-treatment and incubation time for rosette forming cell(RFC) counts were determined. The levels of B-lymphocytes in the PBL were evaluated by erythzocyte-antibody(EA_(Fc))- and erythrocyte-antibody-complement (EAC)-resetting techniques.
The results obtained were as follows;
The PBL from 20 clinically normal Korean cattle were formed as low percentage of spontaneous E-rosette (6.7±2.4%) in control group, whereas in KGRBC treated with 0.1M AET for 20 minutes and 8% Dex were formed as 37.3±2.7% and 45.1±2.1%, respectively. And the synergistic effects were noted no less than 66.5±5.6% when the KGRBC treated with 0.1M AET and 8% Dex subsequently and rate of RFR did not change significantly between 3∼24 hours incubation time at 4℃, EA-and EAC-RFR were 23.3±9.19% and 23.1±7.9%, respectively.
These results suggest that the KGRBC would be a useful agent for the enumeration of T-lymphocytes by E rosette assay and B-lymphocytes by EA- or EAC-rosette assay in cattle-PBL.
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